USA: International Flavors & Fragrances finds fermentation formula for food flavours
The Patent Office employed three examiners (rather than the usual one) to vet this highly technical patent application.
Perfumery is a secretive field, and lactone-based has only three current players, PFW (Netherlands), T. Hasegawa Co. and Givaudan-Roure (International).
A two phase environmentally friendly fermentation process for producing high yields of .delta.-decalactone and .delta.-dodecalactone from the corresponding unsaturated starting materials is carried out under oxidative growth conditions using a saccharomyces species. The resulting products impart, augment and/or enhance the aroma and/or taste of consumable materials including foodstuffs, chewing gums, toothpastes, beverages, fragrance compositions, colognes and perfumed articles such as solid or liquid, anionic, cationic, non-ionic or zwitterionic detergents, fabric softeners, and hair preparations.
First of 20 Claims:
A process for the production of a .delta.-lactone compound represented by the structural formula, wherein R is a member selected from the group consisting of methyl and n-propyl comprising:
(a) preparing an aqueous nutrient medium containing a nutrient source, a buffer and yeast extract at a pH in the range of from about 3.5 up to about 8.0;
(b) preparing as a Saccharomyces yeast culture suspension by inoculating a medium comprising water, a carbon source, a nitrogen source and one or more inorganic salts with a Saccharomyces yeast;
(c) mixing the resulting yeast culture suspension with the aqueous nutrient medium in order to form a first aqueous liquid phase
(d) preparing as a second organic liquid phase an admixture of:
(i) an organic phase former which is a triglyceride of a fatty acid or high molecular weight hydrocarbon; and
(ii) a natural 2-decen-1,5-olide or a natural 2-dodecen-l,5-olide represented by the structural formula, wherein R is selected from the group consisting of methyl and n-propyl;
(e) mixing said first aqueous liquid phase and said second organic liquid phase together with agitation in order to form a reaction medium;
(f) feeding dextrose to said reaction medium at a rate of 5 to 72 g/L/hr so as to maintain said dextrose at a concentration of from 0.01 up to 1.5 grams/liter; and
(g) supplying the reaction medium with an oxygen-containing gas at such a rate as to enable the oxygen level in the reaction medium to be maintained at a level of at least about 0.1 liters/liter of reaction mixture;
By Navroz Havewala, just-food.com correspondent
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